![]() In particular the application of flow cytometry has been instrumental to the diagnosis of primary immunodeficiencies (PIDs) that result from monogenic mutations in key genes of the hematopoietic, and occasionally non-hematopoietic, systems. The flow cytometer has also become a mainstream diagnostic tool in most hospital and pathology laboratories around the world. The advent of flow cytometry has revolutionized the way we approach our research and answer specific scientific questions. 3Clincial Immunogenomics Research Consortium Australia, Darlinghurst, NSW, Australia.Vincent's Clinical School, UNSW Sydney, Sydney, NSW, Australia 1Immunology Division, Garvan Institute of Medical Research, Sydney, NSW, Australia.Moreover, recent progresses in flow cytometry have been discussed in order to give an opinion about the future importance of this technology.Īpoptosis cytokines flow cytometer fluorescence fluorescent-activated cell sorting histogram immunophenotyping light scatter.Cindy S. Additionally, this report provides a basic understanding of flow cytometry technology essential for all users as well as the methods used to analyze and interpret the data. This review covers the general principles and selected applications of flow cytometry such as immunophenotyping of peripheral blood cells, analysis of apoptosis and detection of cytokines. This approach makes flow cytometry a powerful tool for detailed analysis of complex populations in a short period of time. Its working depends on the light scattering features of the cells under investigation, which may be derived from dyes or monoclonal antibodies targeting either extracellular molecules located on the surface or intracellular molecules inside the cell. Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. ![]()
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